遗传学
Yong XIE
发布时间: 2011-05-27   浏览次数: 179

  

Yong XIE
 
Associate Professor, Department of Biology
Hong KongUniversity of Science and Technology
 
Dr Yong Xie's major research interest is focused on protein engineering and his current research projects include:
 
1. Development of an antibody-based vaccine against swine foot-and-mouth disease. FMD is a highly contagious and economically devastating disease affecting cloven-hoofed animals, particularly domestic livestock species such as cattle, goats, and swine. This project explores the possibility of creating an effective and safe FMD vaccine candidate.
 
2. Characterization of a novel mammalian selenocysteine-containing protein (Selenoprotein). Selenoprotein is a protein normally found in patients with auto-immune disease, and cells infected with HIV. This project involves characterization of a newly discovered novel selenoprotein.
 
3. Characterization of a taxol-resistant hepatocellular carcinoma (HCC) cell line. The frequent occurrence of drug resistance of cancer cells to chemotherapeutic drugs usually is a major problem for the treatment of HCC, and drug resistance has been attributed to a number of mechanisms. This project explores the drug resistance mechanism(s) using a taxol-resistant HCC cell line.
 
 
Representative publications:
 
 
EWC Chan, HT Wong, SCS Cheng, ZT Sheng, WY Yan, ZX Zheng, LQ Zhu, Y Xie (2001). An immunoglobulin G based chimeric protein induced foot-and-mouth disease specific immune response in swine. Vaccine 19: 538-546.
 
Kin-Tak Chan, Samuel C-S Cheng, Hong Xie, Yong Xie (2001). A humanized monoclonal antibody constructed from intronless expression vectors targets human hepatocellular carcinoma cells. J Biol Chem. (under review).
 
Yong Xie, Samuel Chak-Sum Cheng, Yi-Wei Huang, Wen Lai, Siu-Hong Chu, Hong Xie (2001). Characterization and mutagenesis of a single-chain Fv recognizing human hepatocellular carcinoma cells. Immunology (under review).
 
Samuel CS Cheng, Dan Luo, Yong Xie (2001). Taxol induced Bcl-2 protein phosphorylation in human hepatocellular carcinoma QGY-7703 cell line. Cell Biol Int. (in press).
 
Jing Zhou, Samuel CS Cheng, Dan Luo, Yong Xie (2001). Study of multi-drug resistant mechanisms in a taxol-resistant hepatocellular carcinoma QGY-TR50 cell line. Biochem Biophy Res Com 280, p1237-1242.
 
Ella WC Chan, Samuel CS Cheng, Yong Xie (2001). Triptolide induced cytotoxic effects on human promyelocytic leukemia, T cell lymphoma and human hepatocellular carcinoma cell lines. Toxicology Letters (in press)..
 
HT Wong, SCS Cheng, EWC Chan, ZT Sheng, WY Yan, ZX Zheng, Y Xie (2000). Plasmid encoding foot-and-mouth disease virus VP1 epitopes elicited immune responses in mice and swine and protected swine against viral infection. Virology 278: 27-35.
 
Xie Y, Zhu LQ, Zhao GP (2000). Assignment of type I phosphatidylinositol-4-phosphate 5-kinase (PIP5K1A) to human chromosome bands 1q22-q24 by in situ hybridization. Cytogenet Cell Genet 88: 197-199.
 
 JH Yuan, RP Zheng, RG Zhang, LX Guo, XW Wang, D Luo, Y Xie, H Xie (2000). Growth-inhibiting effects of taxol on human liver cancer in vitro and in nude mice. World J Gastroentero 6(2): 210-215.
 
Luo Dan, Cheng CS Samuel, Xie Hong, Xie Yong (2000). Effects of Bcl-2 and Bcl-XL protein levels on chemoresistance of hepatoblastoma HepG2 cell line. Biochem Cell Biol 78: 119-126.
 
Luo Dan, Cheng CS Samuel, Xie Yong (1999). Chemosensitivity of human hepatocellular carcinoma cell line QGY-7703 is related to Bcl-2 protein levels. Tumor Biol. 20: 331-340.
 
Luo Dan, Cheng CS Samuel, Xie Yong (1999). Expression of Bcl-2 family proteins during chemotherapeutic agents induced apoptosis in hepatoblastoma HepG2 cell line. Brit J Biomed Sci 56: 114-122.
 
Yang ZJ, Huang HB, Xu QX, Sheng ZT, Xie Y and Zheng ZX (1999). Recombinant fusion protein and DNA vaccine against FMDV infection in guinea-pig and swine. Viral Immunology 12 (1): 1-8.
 
Li HF, Ling MY, Xie Y, Xie H (1998). Establishment of lymph node metastatic model of mouse hepatocellular carcinoma Hca-F cells in C3H/hej mice. Oncogene Res 10: 569-573.
 
Xie Y, Xie H. (1998) A specific murine monoclonal antibody against human hepatocellular carcinoma. Hybridoma 17: 437-444
 
Yang D, Wong MK, Cheung KK, Chan EWC, Xie Y (1997) Study on Triepoxide Analogs of Triptolide, Tetrahedron Letters, 38:6865-6868.
 
Xie Y, Zu MN, Gu JR, Wong P, Wu WQ, Xu GU (1997). Human chromosome pellicle antibody recognizing centromere protein-C (CENP-C), the main component of the kinetochore, Cell Res 7, 13-19.